Assay Kits
Ordering
Name
Cat. No.
Package
Price
500T x 1
9,000 NT
RadiClean
307
500T x 5
35,000 NT
500T x 10
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Detail
Introduction
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Figure 1. The principle of DPPH assay.
Antioxidant capacity is considered as a crucial factor in many human diseases and health impairment. Recent evidences suggest that a diet of antioxidant-rich food, such as vegetables, fruits, barriers, and teas can reduce the risk of various cardiovascular diseases and cancers.
We provided a rapid, simple, and cost-effective product, ReQxi, to evaluate the antioxidant capacity of aqueous and/or alcoholic solutions, such as plant extracts, beverages, serums, etc. As shown in Fig. 1, 2,2-diphenyl-1-picrylhydrazyl (DPPH), a stable compound with free radicals, acts as a scavenger for antioxidant hydrogen radicals. After the absorption of one hydrogen radical, the purple-colored DPPH molecule turns into a colorless DPPH-H. The color change, with an absorbance decrease in 517 nm, is proportional to the antioxidant capacity of the sample solution. Using Trolox as a standard compound, which is included in this assay kit, the antioxidant capacity can be calculated as TEAC (Trolox Equivalent Antioxidant Capacity) value.
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Figure 2. The decrease in OD517 of the standard Trolox solution measured in a 96-well plate.
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Figure 3. The convertion of OD517 into inhibition ratio to obtain IC50.
References
To be Continued...