Assay Kits
Ordering
Name
No.
Package
Price
500T x 1
9,000 TWD
RadiClean
307
500T x 5
35,000 TWD
500T x 10
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Detail
Introduction

Figure 1. The principle of DPPH assay.
Antioxidant capacity is considered as a crucial factor in many human diseases and health impairment. Recent evidences suggest that a diet of antioxidant-rich food, such as vegetables, fruits, barriers, and teas can reduce the risk of various cardiovascular diseases and cancers.
We provided a rapid, simple, and cost-effective product, ReQxi, to evaluate the antioxidant capacity of aqueous and/or alcoholic solutions, such as plant extracts, beverages, serums, etc. As shown in Fig. 1, 2,2-diphenyl-1-picrylhydrazyl (DPPH), a stable compound with free radicals, acts as a scavenger for antioxidant hydrogen radicals. After the absorption of one hydrogen radical, the purple-colored DPPH molecule turns into a colorless DPPH-H. The color change, with an absorbance decrease in 517 nm, is proportional to the antioxidant capacity of the sample solution. Using Trolox as a standard compound, which is included in this assay kit, the antioxidant capacity can be calculated as TEAC (Trolox Equivalent Antioxidant Capacity) value.

Figure 2. The decrease in OD517 of the standard Trolox solution measured in a 96-well plate.

Figure 3. The convertion of OD517 into inhibition ratio to obtain IC50.
References
To be Continued...