Ordering
Name
Cat. No.
Package
Price
CAM-50 μg
287-50 μgx10
50 μg x 10
5,000 NT
CAM-50 mg
287- 50 mg
50 mg
30,000 NT
CAM-100 mg
287-100 mg
100 mg
50,000 NT
Detail
Introduction
Calcein AM can crosses living cell membranes easily. After crossing, it is hydrolyzed by esterase in the cytoplasm to obtain Calcein, which has fluorescent properties and has a highly polar carboxylic acid group, can not penetrate the cell membrane, and remain in the cytoplasm. By measuring Its relative fluorescence intensity, one can calculate the cell viability.
Physical and chemical properties
Appearance : Viscous white to slightly yellow solid.
Solubility :
Soluble in ethyl acetate at room temperature.
10 mg/mL in DMSO at room temperature.
Fluorescence properties : λ ex 496 nm; λ em 516 nm in 0.1 M Tris pH 8.0.
Packaging, Shipping and Storage Information
Packaging: Light-proof plastic or brown glass sealed bottle.
Shipping: Ship at -20 °C.
Storage: Refrigerate at -20 °C for one year in the dark.
Dowload
COA
SDS-English
Chinese MSDS
References
1. Feller, N., et al. 1995. Br. J. Cancer. 72: 543-549. PMID: 7669559
2. Feller, N., et al. 1995. FEBS Lett. 368: 385-388. PMID: 7628644
3. Essodaigui, M., et al. 1998. Biochemistry. 37: 2243-2250. PMID: 9485370
4. Lemasters, JJ, et al. 1998. Biochim. Biophys. Acta. 1366: 177-196. PMID: 9714796
5. Dhar, S., et al. 1998. Eur. J. Pharmacol. 346: 315-322. PMID: 9652375