Assay Kits
Ordering
Name
Cat. No.
Package
Price
500T x 1
3,000 NT
CCK-8
291
500T x 5
13,000 NT
500T x 20
Contact us
500T x 40
Contact us
Detail
Figure 1 Introduction of CCK-8 assay kit
Introduction
The CCK8 assay kit provides a fast, simple and convenient cell viability test. As shown in Figure 1, the light yellow WST8 can be reduced into an orange water-soluble formazan product after a series of redox reactions by intracellular dehydrogenase. The number of living cells is proportional to the absorption of orange WST8 formazan. By detecting the absorbance of formazan (wavelength at 450 nm), the proliferation of living cells can be detected, and then the survival of cells can be analyzed.
Figure 2.The efficiency of patented preservative agent. (days in room temperature)
Surprisingly long shelf life
As Figure 2, this product uses a patented preservative agent, which can slowly release protective gas to lower the background value effectively. Under the storage conditions of -20°C, this product has two-year warranty. We offer a special discount to customers who purchase in bulk. You can order a large number of this prouct to reduce the unit price.
Figure 3. Sensitivity comparison of different assay kits.
More convenient
Traditionally, the MTT kit was used for cell proliferation. After culturing, a water-insoluble formazan product was formed, which accumulated inside the cells and killed the cells. Moreover, after culturing, the supernatant must be removed, and added an organic solvent to dissolve the formazan product before spectral measurement. In contrast, the CCK-8 assay does not require the procedure of removing and adding, which is not only more convenient, but also avoids errors in operation. In addition, the cells are still alive and healthy after measurement, and subsequent experiments such as protein extraction, fluorescent staining, etc. can be continued.
Excellent sensitivity
As shown in Figure 3, we used two cell lines and three different assays to compare at the same time. The sensitivity exhibited by CCK-8 was the best one among these three assays regardless of the cell line. In fact, more and more journal articles have reported the same conclusion with us. Therefore, we sincerely recommend you to use the CCK-8 kit, which can save you a lot of experimental time and labor work.
Chinese instruction manual
Dowload
TDS-English
Chinese MSDS
SDS-English
**If you need a product quality inspection report, please write to us and provide the lot. No. of the product you purchased. **
References
1. Chen, Y.-C.; Chen, Y.-H.; Chiu, H.; Ko, Y.-H.; Wang, R.-T.; Wang, W.-P.; Chuang, Y .-J.; Huang, C.-C.; Lu, T.-T. Cell-Penetrating Delivery of Nitric Oxide by Biocompatible Dinitrosyl Iron Complex and Its Dermato-Physiological Implications. Int. J. Mol. Sci. 2021 , 22, 10101.
2. Sneha Sundaran, Li-Ching Kok and Hwan-You Chang. Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair. Biomedical Materials. 2023.18. 5. 055021.
3. Shun-Ban Tai, Chih-yin Huang, Chih-Ling Chung, Ping-Jyun Sung, Zhi-Hong Wen and Chun-Lin Chen. Prodigiosin Inhibits Transforming Growth Factor β Signaling by Interfering Receptor Recycling and Subcellular Translocation in Epithelial Cells. Molecular Pharmacology April 2024, 105 (4) 286-300.
4 Chen S-H, Wang H-W, Yang P-C, et al. Schwann cells acquire a repair phenotype after assembling into spheroids and show enhanced in vivo therapeutic potential for promoting peripheral nerve repair. Bioeng Transl Med. 2024; 9(2):e10635. doi:10.1002/btm2.10635
5. Chen S-H, Lee Y-W, Kao H-K, et al. The Transplantation of 3-Dimensional Spheroids of Adipose-Derived Stem Cells Promotes Achilles Tendon Healing in Rabbits by Enhancing the Proliferation of Tenocytes and Suppressing M1 Macrophages. The American Journal of Sports Medicine. 2024;52(2):406-422. doi:10.1177/03635465231214698
6. Han Chiu, Anyelina Chau Fang, Yi-Hong Chen, et al. Mechanistic and Kinetic Insights into Cellular Uptake of Biomimetic Dinitrosyl Iron Complexes and Intracellular Delivery of NO for Activation of Cytoprotective HO-1 JACS Au Article ASAP. DOI: 10.1021/jacsau.4c00064