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Ordering
Name
Cat. No.
Package
Price
1 Kit
3,750 NT
CounPro
296
6 Kits
18,750 NT
20 Kits
60,000 NT
Detail
Figure 1. Introduction to the principle of CounPro BCA kit
Introduction
As shown in Figure 1 on the left, in an alkaline environment, proteins bind to copper ions and reduce them to cuprous ions. Quinoline carboxylate ion (BCA) forms a purple complex with cuprous ion. This complex produces an absorption peak at 562 nm in the absorption spectrum. Within the protein concentration range of 20-2000 μg/mL, the light absorption intensity has a linear relationship with the protein con. Proteins can be detected and quantified using this colorimetric method. If the volume of the protein sample is large (0.1 mL), a cuvette can be used for detection; if the volume is small (~10 μL), a 96-well plate can be used.
Product Features
As shown in Figure 2 on the left, for your convenience, this product includes high-purity bovine serum albumin (BSA) standards with the concentrations of 25, 50, 125, 250, 500, 1000, and 2000 μg/mL, respectively. The range of concentration covers the entire linear range for detection. These standards were prepared in large scale to minimize the errors (R square value > 0.99). You don't need to spend time for preparing the standard solution, please use the standard concentration solution directly.
Figure 2. Measurement results of standards included inside the kits.
Dowload
Chinese instruction manual
Chinese MSDS
TDS-English
**If you need a product quality inspection report, please write to us and provide the Lot. number of the product you purchased. **
References
1. Shun-Ban Tai, Chih-yin Huang, Chih-Ling Chung, Ping-Jyun Sung, Zhi-Hong Wen and Chun-Lin Chen. Prodigiosin Inhibits Transforming Growth Factor β Signaling by Interfering Receptor Recycling and Subcellular Translocation in Epithelial Cells. Molecular Pharmacology April 2024, 105 (4) 286-300.