Assay kits
Ordering
Name
Cat. No.
Package
Price
1 Kit
8,999 NT
TAC-Cu
297
6 Kits
44,000 NT
20 Kits
140,000 NT
Detail
Figure 1. A set of ascorbic acid standard calibration lines within the group.
Figure 2. Example of total antioxidant capacity conversion. (A). The results of the same analysis can be presented as OD 570 readings. (B). The total antioxidant capacity of the samples is presented in ascorbic acid equivalent values for comparison between different experiments.
Introduction
Total Antioxidant Capacity (TAC) can represent the antioxidant capacity in the body. Antioxidants play an important role in preventing the formation and scavenging of free radicals and other potentially toxic oxidizing substances. Antioxidants can be divided into three categories: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbic acid, uric acid, vitamin E, etc.) and proteins (albumin, transferrin, etc.). The antioxidant capacity of different antioxidants varies. Detecting TAC in a sample can better reflect the antioxidant status of a sample in a physiological environment than measuring a certain antioxidant alone. Studies have shown that in patients with cancer, diabetes and myocardial infarction, TAC is significantly lower than normal, proving that oxidative stress is an important cause of disease. The measurement results of this set can directly reflect the influence of reactive oxygen species (ROS) in the specimen, and the state of resistance to oxidative damage and oxidative-related diseases.
By removing the interference of GSH and protein signals, this set can measure the antioxidant power exhibited by small molecule antioxidants alone, and reduce Cu2+ to Cu+ through small molecule antioxidants. The reduced Cu+ chelates with the color-forming agent, and produces a strong absorption spectrum change (OD570) near the 570 nm spectrum, which can be quantitatively detected. As shown in Figure 2, by comparing the OD570 of the sample with that of the ascorbic acid standard, the TAC value in the sample can be represented by the antioxidant capacity equivalents of ascorbic acid (ascorbic acid equivalents).
Product Features
This set of technology was transferred from National Tsinghua University and Chang Gung Memorial Hospital, and won the 18th National Innovation Award.
Dowload
Chinese instruction manual
Chinese MSDS
TDS-English
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參考文獻
1. Tsao, Yu‐Ting, et al., Bioeng Transl Med., 2020,6(2), e10199.
2. Tsao, Yu‐Ting, et al., Antioxidants, 2022, 11, 397