Assay kits
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Ordering
Name
Cat. No.
Package
Price
MeasureC-Cu
298
1 Kit
9,990 NT
6 Kits
49,000 NT
20 Kits
150,000 NT
Detail
Figure 1. A set of ascorbic acid standard calibration lines within the group.
Introduction
The detection principle of this set is based on the reduction ability provided by the electrons of antioxidants in the sample, which drives the reduction of cupric ions into cuprous ions, and reacts with the colorant in the set to produce a strong absorption spectrum change at 570 nm. , which can be quantitatively determined. This kit uses ascorbate oxidase (AO) to specifically destroy ascorbic acid, and separates the electron reducing power provided by ascorbic acid in the sample from other antioxidants. Each test sample was assayed in duplicate, one with ascorbate oxidase treatment (+AO) and the other without (-AO). Finally, the ascorbic acid concentration was deduced from the difference in OD570 readings (ΔAO) of the paired samples. This kit requires only a small amount of sample (10 µL), and the limit of detection (LOD) of ascorbic acid concentration can reach 0.016 µM, so it provides a practical analysis tool for the change of ascorbic acid concentration in biological samples.
Set Features
This set of technology was transferred from National Tsinghua University and Chang Gung Memorial Hospital, and won the 18th National Innovation Award.
Dowload
Chinese MSDS
Chinese instruction manual
TDS-English
**If you need a product quality inspection report, please write to us and provide the lot. number of the product you purchased. **
References
1. Tsao, Yu‐Ting, et al., Bioeng Transl Med., 2020, 6(2), e10199.
2. Tsao, Yu‐Ting, et al., Antioxidants, 2022, 11, 397