Assay kits

​Figure 2. Example of total antioxidant capacity conversion. (A).  The results of the same analysis can be presented as OD 570 readings. (B). The total antioxidant capacity of the samples is presented in ascorbic acid equivalent values for comparison between different experiments.

Introduction

Total Antioxidant Capacity (TAC) can represent the antioxidant capacity in the body. Antioxidants play an important role in preventing the formation and scavenging of free radicals and other potentially toxic oxidizing substances. Antioxidants can be divided into three categories: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbic acid, uric acid, vitamin E, etc.) and proteins (albumin, transferrin, etc.). The antioxidant capacity of different antioxidants varies. The detection of TAC in the sample can reflect the antioxidant status of the sample in the physiological environment more than the measurement of a certain antioxidant alone. Studies have shown that TAC is significantly lower in patients with cancer, diabetes and myocardial infarction than normal. They Prove that oxidative stress is an important factor of disease. The measurement results of this set can directly reflect the influence of reactive oxygen species (ROS) in the sample, including the state of resistance to oxidative damage and oxidative-related diseases.

After removing the interference of GSH and protein signals, this set can measure the antioxidant power of sample by reducing Fe3+ to Fe2+. It will produce a strong absorption spectrum change near the 595 nm spectrum which can be quantitatively detected after Fe2+ chelates with the color-forming agent. By comparing the OD595 of the sample with the ascorbic acid standard, the TAC value in the sample can be expressed by ascorbic acid equivalents. This set can use a 96-well plate with a microplate reader or use a 0.5 mL cuvette with the recommended spectrometer for measurement. Please refer to the operation steps for the operation method.