Assay kits

​Figure 1. Ascorbic Acid Standard Curve.

Introduction

The detection theory of this set is based on the reduction ability provided by the electrons of antioxidants in the sample, which drives the reduction of Fe3+ into Fe2+ ions. Fe2+ ions react with the colorant in the set to produce a strong absorption spectrum change at 595 nm. This kit uses ascorbate oxidase (AO) to specifically destroy ascorbic acid and separates the electron reducing power provided by ascorbic acid in the sample from other antioxidants. Each test sample was assayed in duplicate, one with ascorbate oxidase treatment (+AO) and the other without ascorbate oxidase (-AO). Finally, the ascorbic acid concentration was deduced from the difference in OD595 readings (ΔAO) of the paired samples. This kit requires only a small amount of sample (100 µL) and the limit of detection (LOD) of ascorbic acid concentration can reach 0.016 µM. It provides a practical analysis tool for the change of ascorbic acid concentration in biological samples. This set can use a 96-well plate with a microplate reader or use a 0.5 mL cuvette with the recommended spectrometer for measurement. Please refer to the operation steps for the operation method.