Assay Kits

Detail

Introduction

Fig. 1. The reaction of TBA and MDA.

  Malondialdehyde (MDA) is a natural product occurring metabolite of lipid peroxidation. Lipid peroxidation is a mechanism of damage in plant and animal cells and is considered an indicator of oxidative stress in cells and tissues. After peroxidation, polyunsaturated fatty acids (PUFAs) decompose into a complex series of compounds, including the reactive carbonyl compound, MDA. As shown in Fig. 1, the assay method for measuring MDA concentration by reacting with thiobarbituric acid (TBA), is a well-established technique. Numerous researchers have adapted this Thiobarbituric Acid Reactive Substances (TBARS) assay for the evaluation of a variety of sample types, including human and animal tissues, body fluids, pharmaceuticals, and foods. Although there is a debate in the literatures regarding TBA's reactivity with many types of compounds, not just with MDA (e.g., the TBA has the specificity for MDA ?), TBARS remains the most widely used assay for determining lipid peroxidation. If lipoprotein fractions are acid precipitated from the sample first, such as treatment the sample with the Clarificants that provided in this kit (Reagent E), interfering soluble TBARS are minimized, and the test becomes quite specific for lipid peroxidation. In addition, if your ELISA reader has a fluorescent detection function, it is suggested to use fluorescent detection because the fluorescent signal is more sensitive. Meanwhile, as shown in Fig. 1, the 2TBA-MDA product has a special long-chain conjugated structure (highlight with green color), which can emit a unique fluorescent signal, making the fluorescent signal more representative (Toxicol Res. 2014 Mar;30(1):7-11.).

    Fig. 2 shows the calibration curve measured with this TBARS assay kit. Under proper experimental conditions and operation, this assay kit has high sensitivity and reproducibility, making it the best research tool for you.