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Ordering
Name
Cat. No.
Package
Price
1 Kit
2,500 NT
CAMSee
294
6 Kits
12,500 NT
20 Kits
50,000 NT
Detail
Figure 1. Introduction of Calcein AM
Introduction
Calcein AM is a hydrophobic compound that can penetrate membrane of living cell and be hydrolyzed by cell esterase to obtain fluorescent calcein (as shown in Figure 1.). Calcein has highly polar carboxylic acid groups can not penetrate the cell membrane again and remain in the cytoplasm, so we can use to count the number of living cells. CAMsee assay kit is sensitive and reliable, unaffected by redox substances in sample, and can stain and quantify living cells within 1 hour. The Calcein AM Cell Viability Assay can be adapted to a variety of fluorescence devices such as 96-well plate ELISA reader, fluorescence microscopes, and flow cytometers. This assay can be used for a variety of suspension and adherent cell studies, such as cell viability, cell adhesion, chemotaxis, multidrug resistance, apoptosis, and cytotoxicity.
Product Features
As shown in Figure 2, live cells can be observed directly with a microscope; the number of live cells can also be quantified with a fluorescence spectrometer. High sensitivity, accurate quantification as low cell concentration as <100 cells / mL.
Figure 2 Photograph taken by fluorescence microscope after staining cells with our product
Dowload
Chinese MSDS
Chinese instruction manual
TDS-English
**If you need a product quality inspection report, please write to us and provide the Lot. number of the product you purchased. **
References
1. Shun-Ban Tai, Chih-yin Huang, Chih-Ling Chung, Ping-Jyun Sung, Zhi-Hong Wen and Chun-Lin Chen. Prodigiosin Inhibits Transforming Growth Factor β Signaling by Interfering Receptor Recycling and Subcellular Translocation in Epithelial Cells. Molecular Pharmacology April 2024, 105 (4) 286-300.